Measurements of the and final states in the mass range above the and thresholds provide a unique opportunity to measure the off-shell coupling strength of the Higgs boson. This paper presents a determination of the off-shell Higgs boson event yields normalised to the Standard Model prediction (signal strength) in the , and final states. The result is based on collision data collected by the ATLAS experiment at the LHC, corresponding to an integrated luminosity of fb at a collision energy of TeV. Using the method, the observed 95% confidence level (CL) upper limit on the off-shell signal strength is in the range 5.1--8.6, with an expected range of 6.7--11.0. In each case the range is determined by varying the unknown and background K-factor from higher-order QCD corrections between half and twice the value of the known signal K-factor. Assuming the relevant Higgs boson couplings are independent of the energy scale of the Higgs production, a combination with the on-shell measurements yields an observed (expected) 95% CL upper limit on in the range 4.5--7.5 (6.5--11.2) using the same variations of the background K-factor. Assuming that the unknown background K-factor is equal to the signal K-factor, this translates into an observed (expected) 95% CL upper limit on the Higgs boson total width of 22.7 (33.0) MeV.